January 22, 2022

The lab contributed to recent work on RbpA-based transcriptional regulation genome-wide published in the Journal of Bacteriology.

We contributed kinetic and thermodynamic measurements of the effects of mutant RbpA constructs on the equilibrium between open and closed complex. These mutants were used to dissect the genome-wide regulation of mycobacterial transcription in M. tuberculosis. Interestingly, RbpA mutants led to both up-regulation or down-regulation in a promoter specific manner suggesting the ability of RbpA to either repress or activate genes depending on the context.

Click here to link to the paper at the Journal of Bacteriology.