January 22, 2022

Flux Calculator

The lab has developed online calculators that allow for the direct testing of mechanistic hypotheses by calculating the steady-state transcript flux in the presence and absence of a factor as a function of initiation rate constants. By evaluating how the flux ratio of a single kinetic mechanism varies across promoter space, quantitative insights into the potential of a mechanism to generate promoter-specific regulatory outcomes can be obtained.

Click here for more information and to access the calculators on github.

Click here to read the published work describing the calculators in detail and applying them to two important transcription factors in E. coli and M. tuberculosis.