2025
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Oligomerisation of Ku from Mycobacterium tuberculosis promotes DNA synapsis.
Zahid S, Baconnais S, Smith H, Atwal S, Bates L, Read H, Chadda A, Morati F, Bedwell T, Stender EGP, Walter J, Hardwick S, Westerlund F, Galburt EA, Le Cam E, Pyne A, Mukamolova G, Chaplin A
Nature Communications, 2025 Nov 26
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Serine/threonine protein kinase phosphorylation of DosR alters target gene transcription mechanics and regulates Mycobacterium tuberculosis sensitivity to nitric oxide stress.
Natalie R. Sontag, Ana Ruiz Manzano, Alwyn M.V. Ecker, Eric A. Galburt, and Shumin Tan
bioRxiv, 2025 Sep 3
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Regulation of steady state ribosomal transcription in Mycobacterium tuberculosis: Intersection of sigma subunits, superhelicity and transcription factors.
Ana Ruiz Manzano, Drake Jensen, and Eric A. Galburt
Journal of Biological Chemistry. 2025 Aug; 301(8):110369
Structural basis of dimerization and activation of UvrD-family helicases.
Ankita Chadda, Binh Nguyen, Timothy Lohman, and Eric A. Galburt
PNAS. 2025 Mar 11;122(10):e2422330122.
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Mycobacterium tuberculosis Ku stimulates multi-round DNA unwinding by UvrD1 monomers.
Ankita Chadda, Alex Kozlov, Binh Nguyen, Timothy Lohman, and Eric A. Galburt
Journal of Molecular Biology. Volume 436 Issue 2, 15 Jan 2024.
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High-throughput, aptamer-based-measurements of steady state transription rates for Mycobacterium tuberculosis RNA polymerase
Drake Jensen, Ana Ruiz Manzano, Max Rector, Eric J. Tomko, M. Thomas Record, and Eric A. Galburt
Nucleic Acids Research. Volume 51 Issue 19, 30 Oct 2023.
2024
2023
Shelterin Components Modulate Nucleic Acid Condensation and Phase Separation in the Context of Telomeric DNA
Andrea Soranno, J. Jeremías Incicco, Paolo De Bona, Eric J. Tomko, Eric A. Galburt, Alex S. Holehouse, and Roberto Galletto
JMB. Volume 434, Issue 16, 30 Aug 2022, 167685.
2022
Parallel path mechanisms lead to nonmonotonic force-velocity curves and an optimum load for molecular motor fucntion
Upasana Mallimadugula and Eric A. Galburt
Physical Review E. 2022 Mar:105(3-1):034405.
2021
2020
2019
CarD contributes to diverse gene expression outcomes throughout the genome of Mycobacterium tuberculosis.
Dennis Zhu, Ashley Garner, Eric A. Galburt, and Christina Stallings
Proceedings of the National Academy of Sciences. 2019 Jul 2; 116(27): 13573–13581.
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CarD and RbpA modify the kinetics of initial transcription and slow promoter escape of the Mycobacterium tuberculosis RNA polymerase
Drake Jensen, Ana Ruiz Manzano, Jayan Rammohan, Christina Stallings, and Eric A. Galburt
Nucleic Acids Research 2019 Jul 26;47(13):6685-6698.
Single-molecule approach for studying RNAP II transcription initiation using magnetic tweezers.
Tomko EJ and Galburt EA
Methods. 2019 Apr 15;159-160:35-44.
2018
The calculation of transcript flux ratios reveals single regulatory mechanisms capable of activation and repression.
Galburt EA
Proceedings of the National Academy of Sciences. 2018 Dec 11;115(50):E11604-E11613.
Domains within RbpA serve specific functional roles that regulate the expression of distinct mycobacterial gene subsets.
Prusa J, Jensen D, Santiago-Collazo G, Pope SS, Garner AL, Miller JJ, Ruiz Manzano A, Galburt EA, and Stallings CL
Journal of Bacteriology. 2018 April;Jul 1; 200(13): e00690-17.
2017
- TFIIH generates a six-base-pair open complex during RNAP II transcription initiation and start-site scanning.
Tomko EJ, Fishburn J, Hahn S, and Galburt EA
Nature Structural and Molecular Biology. 2017 Dec;24(12):1139-1145.
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Conformational selection and induced fit as a useful framework for molecular motor mechanisms.
Galburt EA and Tomko EJ
Biophysical Chemistry. 2017 April; 223:11-16.
Effects of increasing the affinity of CarD for RNA Polymerase on Mycobacterium tuberculosis Growth, rRNA Transcription, and Virulence.
Garner AL, Rammohan J, Huynh JP, Onder LM, Chen J, Bae B, Jensen D, Weiss LA, Ruiz Manzano A, Darst SA, Campbell EA, Nickels BE, Galburt EA, Stallings CL
Journal of Bacteriology. 2017 January; 199(4) pii:e00698-16.
2016
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A kinetic signature for parallel pathways: conformational selection and induced fit.
Galburt EA and Rammohan J
Biochemistry. 2016 December; 55(50):7014-7022.
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Cooperative stabilization of Mycobacterium tuberculosis rrnAP3 promoter open complexes by RbpA and CarD.
Rammohan J, Ruiz Manzano A, Garner AL, Prusa J, Stallings CL, Galburt EA
Nucleic Acids Research. 2016 July; 44(15):7304-7313.
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Transcription Start Site Scanning and the Requirement for ATP During Transcription Initiation by RNA Polymerase II.
Fishburn J, Galburt EA, Hahn S
Journal of Biological Chemistry. 2016 April; 291(25):13040-13047.
- Mechanisms of backtrack recovery by RNA polymerases I and II.
Lisica A, Engel C, Jahnel M, Roldán É, Galburt EA, Cramer P, Grill SW
PNAS. 2016 February; 113(11):2946-2951.
2015 and prior
Double-stranded DNA translocase activity of TFIIH and the mechanism of RNA Polymerase II Open Complex formation.
Fishburn J, Tomko EJ, Galburt EA, Hahn S
PNAS. 2015 March; 112(13):3961-6.
CarD stabilizes mycobacterial open complexes via a two-tiered kinetic mechnansim.
Rammohan J, Ruiz Manzano A, Garner AL, Stallings CL, Galburt EA
Nucleic Acids Research. 2015 February; 43(6):3272-85.
Selected by reviewers and editors as a NAR Breakthrough Article.
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CarD intergrates three functional modules to promote efficient transcription, antibiotic resistance, and pathogenesis in mycobacteria.
Garner AL, Weiss LA, Ruiz Manzano A, Galburt EA, Stallings CL
Molecular Microbiology. 2014 July; 93(4):682-97.
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Force-dependent melting of supercoiled DNA at thermophilic temperatures.
Galburt EA, Tomko EJ, Stump W, Ruiz Manzano A
Biophysical Chemistry. 2014 March; (187-8):23-28.
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Magnetic tweezers force spectroscopy.
Galburt EA
Handbook of Imaging in Biological Mechanics
Editors: Corey Neu and Guy Genin
CRC Press, Boca Raton, 2004. -
RNA polymerase pushing.
Galburt EA, Parrondo JMR, Grill SW
Biophysical Chemistry. 2011 August; 157(1-3):43-7. -
The origin of short transcriptional pauses.
Depken M, Galburt EA, Grill SW
Biophysical Journal. 2009 March; 96(6):2189-93. -
Single Molecule Transcription Elongation.
Galburt EA, Grill SW, Bustamante C.
Methods. 2009 Aug; 48(4):323-32. -
Backtracking determines the force sensitivity of RNAP II.
Galburt EA, Grill SW, Weidmann A, Lubkowska L, Choy J, Nogales E, Kashlev M, Bustamante CJ
Nature. 2007 April; 446(7137):820-3. -
His-Cys Box Homing Endonucleases.
Galburt EA and Jurica MS
Homing Endonucleases and Inteins. Editors: Belfort M, Stoddard BL, Wood DW, and Derbyshire V.
Springer-Verlag, Berlin Heidelberg, 2005. -
Structure of a tRNA repair enzyme and molecular biology workhorse: T4 polynucleotide kinase.
Galburt EA, Pelletier J, Wilson G, Stoddard BL
Structure. 2002 September; 10(9):1249-60. -
Catalytic mechanisms of restriction and homing endonucleases.
Galburt EA and Stoddard BL.
Biochemistry. 2002 Nov; 41(47):13851-60. -
Conformational changes and cleavage by the homing endonuclease I-PpoI: A critical role for a leucine residue in the active site.
Galburt EA, Chadsey MS, Jurica MS, Chevalier BS, Erho D, Tang W, Monnat RJ Jr., Stoddard BL
Journal of Molecular Biology. 2002 Jul; 300(4):877-87. -
Restriction endonucleases: one of these things is not like the others.
Galburt EA and Stoddard BL.
Nature Structural Biology 2000 Feb; 7(2):89-91. -
A novel endonuclease mechanism directly visualized for I-PpoI.
Galburt EA, Chevalier BS, Tang W, Jurica MS, Flick KE, Monnat RJ Jr., Stoddard BL
Nature Structural Biology. 1999 December; 6(12):1096-99.