Click here to see how Eric Tomko uses magnetic tweezers assays to describe mechanisms of DNA unwinding by the pre-initiation complex of S. cerevisiae.
The lab’s new resource for performing calculations of regulated transcriptional flux ratios across promoter space has been published in PNAS. Click here to see the paper.
Our results obtained by monitoring DNA bubble size with magnetic tweezers constrain possible mechanisms of DNA unwinding and reveal that DNA unwinding occurs in two separate steps. An initial step catalyzed by TFIIH leads to the melting of 6 bp while subsequent initial transcription by RNAP II expands the bubble to 15 bp. Click here […]