We contributed kinetic and thermodynamic measurements of the effects of mutant RbpA constructs on the equilibrium between open and closed complex. These mutants were used to dissect the genome-wide regulation of mycobacterial transcription in M. tuberculosis. Interestingly, RbpA mutants led to both up-regulation or down-regulation in a promoter specific manner suggesting the ability of RbpA to either repress or activate genes depending on the context.
Click here to link to the paper at the Journal of Bacteriology.